Experimental Study on the Effective Production of Biocement for Soil Solidification and Wind Erosion Control

Biocement can be achieved through the microbially induced carbonate precipitation (MICP) process. Such a method potentially utilized as an eco-friendly for civil and environmental engineering applications such soil ground improvement wind erosion control of surface soil. In this method, one key step is effective production ureolytic bacteria. previous laboratory field studies, cultivation bacteria used MICP were usually expensive time-consuming. The purpose study was to optimize (Sporosarcina pasteurii), stabilization tests conducted verify effectiveness cultured strengthen against wind-induced erosion. Bacterial methods studied by investigating effects different media conditions. Testing variables included types concentrations nitrogen sources (urea or NH4Cl), pH values (7.5–9.5), conditions (batch chemostat condition), carbon sources. It found that, with same amount source, test pure urea had highest biomass yield, urease activity, specific activity than other NH4Cl both urea. use source in also led increase pH, which not NH4Cl. As factor concentration, higher concentration pH. played important role. 8.5 initial value 7.5 9.5 values. condition, could effectively produced up 7 mmol/L/min, compared around 12 mmol/L/min batch condition. Thus, optimum value, condition Sporosarcina pasteurii urea, 8.5, respectively. addition, when soybean milk powder 2.5 high enough biocement.